### General information Author: xxx Anna Rosling, https://www.ieg.uu.se/evolutionary-biology/rosling/ Contact e-mail: anna.rosling@ebc.uu.se License: CC BY 4.0 This readme file was last updated: 23-08-2022 Please cite as: Eshghi Sahraei et al (2022) ### Dataset description The study presents whole genome analysis of data generated from two spores of Funneliformis geosprum, collected at Kungsängen Nature reserve in southern Uppsala, Sweden. The file "Spore nuclie scatter plots and MDA results for ..." contains information on the spores from which nuclei were sorted in this project including FACS scatterplot, well positions, MDA, PCR screening for bacteria and fungi as well as a list of which nuclei were selected for sequening. The zipped folder "Alignments for species identification.zip" contains alignments and phylogenetic trees for there reference genes (Fox, LSU and Tor) used for species identification of the morphotyped spores agains known species. The file "Alignment of MAT contigs from all funneliformis.phy" contains one contig from each of the four Funneliformis genome assemblies with a gene homologous to one of the genes in the mating type locus identified in Rhizophagus irregulars, gene bank accession number KT946687 The file "Alignment of MAT contigs from all funneliformis extraction.fasta" contains the region from each of the four contigs that was extracted from the above alignment and used to Blast all single nuclei assemblies for potential mating type locus homologues. The two excel files "Fig 3a..." and "Fig 3b..." contains the FunAnnotate output for gene families CAZyme and MEROPS and calculations for Fig 3a and b.