### General information

Author: Joakim Klar
Contact e-mail: joakim.klar@igp.uu.se
DOI: 10.17044/scilifelab.20472144
License: CC BY 4.0
This readme file was last updated: 12-08-2022

Please cite as: Klar, Joakim (2022): Data: Gene expression of Mowat-Wilson syndrome patient derived GABAergic interneurons SciLifeLab. Dataset. https://doi.org/10.17044/scilifelab.20472144

### Dataset description

RNA from iPSC and neural cell populations was isolated using an miRNeasy micro kit (Qiagen).

IPSC lines established from fibroblasts of both sibling (MW1=26, MW2=27) and two healthy donors (Ctl2=K02 and Ctl8=K08).

Neurocortical differentiation of iPSCs (samples IPS) was carried out as described using Dual-SMAD inhibition that promotes conversion into neural stem cells (NSC) for 10 days (samples INT) followed by directed differentiation into cortical lineages for 65 days (samples D65).

Bulk RNA sequencing reads were aligned to the ENSEMBL human reference genome (Homo_sapiens.GRCh37.75) and gene counts were generated using the STAR read aligner. The count data is not normalized and can be used with DESEq2.

The uploaded data consist of count data and can be used for gene expression analysis of patients and controls.

Expression data was generated for all three time points of differentiation into GABAergic interneurons: Puripotent stage (IPS) -> INT (D10) -> D65

File names are:
timepoint_SampleReadsPerGene.out.tab