2D MSE Errors after correction (pixels): Cy5 a488 a594 a700 dapi tmr ________ ________ ________ ________ ________ ________ Cy5 0 0.049346 0.044798 0.034484 0.074294 0.042912 a488 0.047954 0 0.070608 0.045883 0.033465 0.012541 a594 0.074396 0.10275 0 0.065797 0.084508 0.09572 a700 0.043867 0.062194 0.0463 0 0.09308 0.077077 dapi 0.082109 0.036333 0.089521 0.085179 0 0.069708 tmr 0.042532 0.012037 0.083611 0.057877 0.059929 0 3D MSE Errors after correction (pixels): Cy5 a488 a594 a700 dapi tmr ________ ________ _______ ________ _______ ________ Cy5 0 0.07978 0.1181 0.091107 0.31818 0.13115 a488 0.077814 0 0.18113 0.091602 0.13537 0.053301 a594 0.18237 0.2295 0 0.2214 0.27202 0.24169 a700 0.10206 0.11 0.15678 0 0.37867 0.19644 dapi 0.32574 0.25592 0.28358 0.36465 0 0.30096 tmr 0.13019 0.052627 0.16888 0.17294 0.28873 0 Errors before correction: Cy5 a488 a594 a700 dapi tmr _______ _______ ______ _______ _______ _______ Cy5 0 1.3024 7.5582 0.63152 0.54487 0.70875 a488 1.3134 0 5.4593 1.1406 0.90799 0.20727 a594 7.6711 5.3787 0 4.1095 4.3428 4.8485 a700 0.62282 1.1311 4.0321 0 0.15133 0.65966 dapi 0.5432 0.82173 4.3853 0.13418 0 0.40242 tmr 0.71301 0.20121 4.8099 0.59346 0.37098 0 Displacement length in centre (pixels): Cy5 a488 a594 a700 dapi tmr ________ ________ ________ ________ ________ ________ Cy5 {'0.00'} {'0.85'} {'2.74'} {'0.79'} {'1.10'} {'0.61'} a488 {'0.85'} {'0.00'} {'2.43'} {'0.73'} {'1.64'} {'0.46'} a594 {'2.76'} {'2.41'} {'0.00'} {'1.97'} {'2.31'} {'2.22'} a700 {'0.77'} {'0.74'} {'1.97'} {'0.00'} {'1.07'} {'0.34'} dapi {'1.10'} {'1.54'} {'2.32'} {'1.08'} {'0.00'} {'1.22'} tmr {'0.61'} {'0.46'} {'2.22'} {'0.31'} {'1.22'} {'0.00'} Displacement in centre (pixels): Cy5 a488 a594 a700 dapi tmr _________________ _________________ __________________ __________________ _________________ __________________ Cy5 {'[0.00, 0.00]' } {'[-0.39, 0.09]'} {'[-2.74, 0.07]' } {'[-0.78, -0.01]'} {'[-0.63, 0.12]'} {'[-0.52, 0.06]' } a488 {'[0.39, -0.09]'} {'[0.00, 0.00]' } {'[-2.36, -0.01]'} {'[-0.41, -0.09]'} {'[-0.34, 0.01]'} {'[-0.17, -0.03]'} a594 {'[2.75, -0.09]'} {'[2.33, 0.02]' } {'[0.00, 0.00]' } {'[1.97, -0.08]' } {'[2.06, 0.04]' } {'[2.21, -0.01]' } a700 {'[0.75, 0.01]' } {'[0.43, 0.09]' } {'[-1.96, 0.07]' } {'[0.00, 0.00]' } {'[0.18, 0.13]' } {'[0.28, 0.07]' } dapi {'[0.62, -0.12]'} {'[-0.09, 0.04]'} {'[-2.07, -0.04]'} {'[-0.15, -0.13]'} {'[0.00, 0.00]' } {'[0.11, -0.08]' } tmr {'[0.52, -0.05]'} {'[0.17, 0.03]' } {'[-2.21, 0.01]' } {'[-0.26, -0.07]'} {'[-0.10, 0.08]'} {'[0.00, 0.00]' } Axial displacement (z, in pixels): Cy5 a488 a594 a700 dapi tmr ____________ ____________ ____________ ____________ ____________ ____________ Cy5 {0×0 double} {[ 0.7484]} {[ 0.1398]} {[ 0.1530]} {[ -0.8941]} {[ 0.3204]} a488 {[ -0.7481]} {0×0 double} {[ -0.6114]} {[ -0.5903]} {[ -1.6032]} {[ -0.4305]} a594 {[ -0.1407]} {[ 0.6152]} {0×0 double} {[ -0.0017]} {[ -1.0401]} {[ 0.1804]} a700 {[ -0.1497]} {[ 0.5938]} {[ -0.0078]} {0×0 double} {[ -1.0499]} {[ 0.1693]} dapi {[ 0.8999]} {[ 1.5332]} {[ 1.0442]} {[ 1.0606]} {0×0 double} {[ 1.2159]} tmr {[ -0.3202]} {[ 0.4290]} {[ -0.1894]} {[ -0.1661]} {[ -1.2097]} {0×0 double} Number of dots: Cy5 a488 a594 a700 dapi tmr ___ ____ ____ ____ ____ ___ Cy5 0 118 153 158 144 143 a488 121 0 122 102 51 125 a594 155 120 0 140 143 158 a700 162 101 142 0 137 137 dapi 155 52 153 143 0 143 tmr 144 122 157 133 133 0