2D MSE Errors after correction (pixels): Cy5 a488 a594 a700 dapi tmr _______ _______ _______ _______ _______ _______ Cy5 0 0.19202 0.18699 0.22522 0.19595 0.2475 a488 0.20257 0 0.26072 0.41308 0.14128 0.23618 a594 0.21717 0.27546 0 0.23881 0.14311 0.23497 a700 0.21196 0.27587 0.22706 0 0.20044 0.21249 dapi 0.21082 0.16639 0.20552 0.22156 0 0.18225 tmr 0.16118 0.28137 0.22086 0.22597 0.1356 0 3D MSE Errors after correction (pixels): Cy5 a488 a594 a700 dapi tmr _______ _______ _______ _______ ______ _______ Cy5 0 1.1977 0.56977 0.88638 1.3883 0.6007 a488 1.0814 0 0.76634 0.96731 1.1538 0.56 a594 0.6592 0.90636 0 1.0042 1.3546 0.46061 a700 0.98347 1.2072 1.2144 0 1.7152 0.92929 dapi 1.6279 1.453 1.6564 1.7068 0 1.5695 tmr 0.51926 0.67279 0.39595 0.74109 1.3436 0 Errors before correction: Cy5 a488 a594 a700 dapi tmr _______ ______ ______ _______ _______ ______ Cy5 0 2.3567 7.4592 0.88513 0.95636 1.1882 a488 2.1159 0 8.6963 3.2252 2.3351 1.0828 a594 7.3498 9.4052 0 4.6819 3.9189 4.9302 a700 0.84543 3.1857 4.6634 0 0.79259 1.1981 dapi 0.87478 2.1636 3.8546 0.70262 0 0.5113 tmr 1.079 1.0768 4.9451 1.1541 0.40693 0 Displacement length in centre (pixels): Cy5 a488 a594 a700 dapi tmr ________ ________ ________ ________ ________ ________ Cy5 {'0.00'} {'0.80'} {'2.68'} {'1.06'} {'0.72'} {'0.80'} a488 {'0.75'} {'0.00'} {'2.46'} {'0.68'} {'0.98'} {'0.58'} a594 {'2.59'} {'2.45'} {'0.00'} {'2.10'} {'2.03'} {'2.11'} a700 {'1.04'} {'0.76'} {'2.03'} {'0.00'} {'0.91'} {'0.63'} dapi {'0.67'} {'1.03'} {'2.04'} {'1.00'} {'0.00'} {'0.54'} tmr {'0.74'} {'0.60'} {'2.09'} {'0.67'} {'0.51'} {'0.00'} Displacement in centre (pixels): Cy5 a488 a594 a700 dapi tmr _________________ __________________ __________________ __________________ _________________ _________________ Cy5 {'[0.00, 0.00]' } {'[-0.42, -0.25]'} {'[-2.67, 0.15]' } {'[-0.81, -0.40]'} {'[-0.66, 0.22]'} {'[-0.72, 0.15]'} a488 {'[0.35, 0.14]' } {'[0.00, 0.00]' } {'[-2.39, 0.17]' } {'[-0.58, -0.35]'} {'[-0.32, 0.36]'} {'[-0.37, 0.13]'} a594 {'[2.59, -0.15]'} {'[2.39, -0.18]' } {'[0.00, 0.00]' } {'[1.99, -0.52]' } {'[2.01, 0.09]' } {'[2.10, -0.14]'} a700 {'[0.81, 0.36]' } {'[0.62, 0.43]' } {'[-1.92, 0.52]' } {'[0.00, 0.00]' } {'[0.17, 0.59]' } {'[0.16, 0.56]' } dapi {'[0.60, -0.23]'} {'[0.36, -0.32]' } {'[-2.00, -0.09]'} {'[-0.13, -0.61]'} {'[0.00, 0.00]' } {'[0.03, -0.16]'} tmr {'[0.67, -0.11]'} {'[0.42, -0.09]' } {'[-2.08, 0.15]' } {'[-0.13, -0.60]'} {'[-0.07, 0.18]'} {'[0.00, 0.00]' } Axial displacement (z, in pixels): Cy5 a488 a594 a700 dapi tmr ____________ ____________ ____________ ____________ ____________ ____________ Cy5 {0×0 double} {[ 0.6316]} {[ 0.1723]} {[ 0.5410]} {[ -0.1724]} {[ 0.3165]} a488 {[ -0.6468]} {0×0 double} {[ -0.5429]} {[ -0.0383]} {[ -0.8506]} {[ -0.4281]} a594 {[ -0.1484]} {[ 0.5252]} {0×0 double} {[ 0.4071]} {[ -0.3004]} {[ 0.1527]} a700 {[ -0.5485]} {[ 0.0431]} {[ -0.3762]} {0×0 double} {[ -0.6752]} {[ -0.2419]} dapi {[ 0.1847]} {[ 0.9038]} {[ 0.3561]} {[ 0.7765]} {0×0 double} {[ 0.5142]} tmr {[ -0.2964]} {[ 0.4261]} {[ -0.1403]} {[ 0.2619]} {[ -0.4771]} {0×0 double} Number of dots: Cy5 a488 a594 a700 dapi tmr ___ ____ ____ ____ ____ ___ Cy5 0 63 144 134 102 144 a488 70 0 125 68 61 135 a594 144 116 0 139 100 143 a700 146 68 149 0 106 140 dapi 114 73 114 109 0 112 tmr 140 135 145 131 100 0