2D MSE Errors after correction (pixels): Cy5 a488 a594 a700 dapi tmr ________ ________ ________ ________ ________ ________ Cy5 0 0.072269 0.085792 0.059182 0.12085 0.037266 a488 0.070481 0 0.068926 0.091623 0.067304 0.04724 a594 0.12106 0.072695 0 0.10027 0.099448 0.04363 a700 0.075103 0.089551 0.082142 0 0.12208 0.045888 dapi 0.12117 0.10825 0.11004 0.12715 0 0.1436 tmr 0.084006 0.048031 0.048811 0.075803 0.10303 0 3D MSE Errors after correction (pixels): Cy5 a488 a594 a700 dapi tmr _______ _______ _______ _______ _______ _______ Cy5 0 0.11492 0.11071 0.12318 0.41114 0.12391 a488 0.1185 0 0.143 0.171 0.51585 0.11487 a594 0.18698 0.14656 0 0.28102 0.40754 0.13696 a700 0.14428 0.16867 0.14937 0 0.44577 0.15173 dapi 0.41321 0.61399 0.41044 0.46253 0 0.35268 tmr 0.17333 0.11594 0.11421 0.18293 0.30516 0 Errors before correction: Cy5 a488 a594 a700 dapi tmr _______ _______ ______ _______ _______ _______ Cy5 0 1.6236 6.7872 0.65019 0.31872 0.68726 a488 1.6739 0 5.2011 1.6373 1.6095 0.26348 a594 6.9251 5.2238 0 3.7208 4.9475 4.5015 a700 0.68067 1.6336 3.6778 0 0.32562 0.69454 dapi 0.3135 1.4388 5.0587 0.33603 0 0.54548 tmr 0.71729 0.26149 4.5063 0.69053 0.49288 0 Displacement length in centre (pixels): Cy5 a488 a594 a700 dapi tmr ________ ________ ________ ________ ________ ________ Cy5 {'0.00'} {'0.88'} {'2.57'} {'0.86'} {'1.19'} {'0.60'} a488 {'0.87'} {'0.00'} {'2.37'} {'0.59'} {'1.71'} {'0.50'} a594 {'2.59'} {'2.38'} {'0.00'} {'1.86'} {'2.47'} {'2.07'} a700 {'0.85'} {'0.59'} {'1.86'} {'0.00'} {'1.56'} {'0.27'} dapi {'1.20'} {'1.78'} {'2.49'} {'1.58'} {'0.00'} {'1.44'} tmr {'0.62'} {'0.50'} {'2.05'} {'0.27'} {'1.43'} {'0.00'} Displacement in centre (pixels): Cy5 a488 a594 a700 dapi tmr _________________ _________________ __________________ __________________ _________________ __________________ Cy5 {'[0.00, 0.00]' } {'[-0.35, 0.06]'} {'[-2.57, 0.02]' } {'[-0.73, -0.12]'} {'[-0.46, 0.06]'} {'[-0.49, 0.05]' } a488 {'[0.34, -0.06]'} {'[0.00, 0.00]' } {'[-2.26, -0.06]'} {'[-0.41, -0.19]'} {'[0.41, -0.49]'} {'[-0.18, -0.04]'} a594 {'[2.59, -0.00]'} {'[2.27, 0.06]' } {'[0.00, 0.00]' } {'[1.83, -0.14]' } {'[2.16, 0.06]' } {'[2.06, 0.01]' } a700 {'[0.72, 0.11]' } {'[0.41, 0.18]' } {'[-1.83, 0.12]' } {'[0.00, 0.00]' } {'[0.27, 0.19]' } {'[0.21, 0.14]' } dapi {'[0.45, -0.05]'} {'[-0.40, 0.64]'} {'[-2.19, -0.07]'} {'[-0.30, -0.20]'} {'[0.00, 0.00]' } {'[-0.10, -0.05]'} tmr {'[0.51, -0.03]'} {'[0.19, 0.04]' } {'[-2.04, 0.00]' } {'[-0.22, -0.13]'} {'[0.09, 0.05]' } {'[0.00, 0.00]' } Axial displacement (z, in pixels): Cy5 a488 a594 a700 dapi tmr ____________ ____________ ____________ ____________ ____________ ____________ Cy5 {0×0 double} {[ 0.8010]} {[ 0.0909]} {[ 0.4374]} {[ -1.0993]} {[ 0.3496]} a488 {[ -0.8007]} {0×0 double} {[ -0.7116]} {[ -0.3814]} {[ -1.5847]} {[ -0.4638]} a594 {[ -0.0969]} {[ 0.7180]} {0×0 double} {[ 0.3302]} {[ -1.1878]} {[ 0.2537]} a700 {[ -0.4368]} {[ 0.3830]} {[ -0.3362]} {0×0 double} {[ -1.5274]} {[ -0.0854]} dapi {[ 1.1079]} {[ 1.6153]} {[ 1.1914]} {[ 1.5352]} {0×0 double} {[ 1.4323]} tmr {[ -0.3507]} {[ 0.4636]} {[ -0.2471]} {[ 0.0860]} {[ -1.4251]} {0×0 double} Number of dots: Cy5 a488 a594 a700 dapi tmr ___ ____ ____ ____ ____ ___ Cy5 0 96 130 137 138 130 a488 98 0 109 82 19 121 a594 136 112 0 127 140 159 a700 142 89 123 0 123 125 dapi 144 22 144 127 0 125 tmr 131 119 155 123 117 0