### General information 


Author: Maja Malmberg, Mette Myrmel, Mamata Khatri
Contact e-mail: maja.malmberg@slu.se
DOI:10.17044/scilifelab.14377097
License: CC BY 4.0
This readme file was last updated: 06-04-2021

Please cite as: Dataset of SARS-CoV-2 wastewater data from Örebro, Sweden (2021). 10.17044/scilifelab.14377097

### Dataset description

This is a metadata record for a continuously updated dataset of SARS-CoV-2 RNA data in wastewater in Örebro, Sweden.

The dataset is part of a research study led by associate professor Maja Malmberg (SLU, Swedish University of Agricultural Sciences) and her research group in collaboration with associate professor Mette Myrmel at the Norwegian University of Life Sciences. The amount of SARS-CoV-2 virus is measured in wastewater from the wastewater treatment facility in Örebro. The research group is part of the Environmental Virus Profiling Research Area of the SciLifeLab National COVID-19 Research Program.

After preparation, the viruses are extracted using ultra filtration and analyzed using qPCR technique for SARS CoV-2 RNA. RT-qPCR samples are normalized against Pepper Mild Mottle Virus (PMMoV). Until January 2021, three samples per week were taken and an average of the three values was calculated to provide a weekly estimate. From February to March 2021, samples were only collected at one time point per week, and from April onwards two samples per week are analysed and the average presented. Primers used to detect the SARS-COV-2 gene have been previously used and verified by Corman and colleagues, 2020.

For more information see https://www.covid19dataportal.se/data_types/environment/wastewater/ The dataset started week 44 2020 and updates every second week.

The dataset is available as part of the Environmental Virus Profiling data section "The amount of SARS-CoV-2 virus in wastewater across Sweden" https://www.covid19dataportal.se/data_types/environment/wastewater/#orebro

For more information see https://www.covid19dataportal.se/data_types/environment/wastewater/ The dataset started week 44 2020 and updates weekly but will be added to the Portal monthly.

The dataset is available as part of the Environmental Virus Profiling data section "The amount of SARS-CoV-2 virus in wastewater across Sweden" https://covid19dataportal.se/data_types/environment/wastewater/#orebro on the Swedish COVID-19 Data Portal (https://covid19dataportal.se ).
Keywords: Covid-19 data, environmental monitoring, wastewater outlet, RNA amount, SARS-CoV-2

All nr are change in amount of SARS CoV-2 RNA (%) compared to Nov 6 2020 standardized to flow, showed each week. 

Variables in Excel
A Vecka Week the sample was collected
B Datum the date the sample was collected
C Plats Place sample was collected
D SARS CoV-2 (%) Relative amount of SARS CoV-2 (%)
E Medelvärde per vecka mean of samples collected the same week
J Vecka week
K SARS CoV-2 (%) compare to amount SARS CoV-2 Nov 6th 2020 in Örebro.

The virus copies are standardized to bovine coronavirus (BCoV) and Pepper Mild Mottle Virus (PMMoV) (%)