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Simultaneous visualization of DNA loci in single cells by combinatorial multi-color iFISH (Dataset 3: miFISH with one dual-color probe AF594-AF488 and two single-color probes AT542 and AT647N)

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posted on 18.06.2021, 10:04 by Ana Faustino MotaAna Faustino Mota, Maud Schweitzer, Magda BienkoMagda Bienko
We performed a single colour experiment where one probe with alternated colours (AF488-AF594) was tested together with other two probes in different colours located in distinct genomic sites. There are 1 probe with AF488 dye, 1 probe with AT542, 1 probe with AF594 and 1 probe with AT647N.

Experiment Imaging Method: epifluorescence microscopy
Protocol Name: iFISH protocol

Each dataset consists of multiple fields of view (FOVs) that were acquired with seven different dyes (AF488 labeled as ‘a488’; AT542 as ‘tmr’; AF594 as ‘a594’; AT647N as ‘Cy5’, AF700 as ‘a700’, AF790 as ‘ir800’; and Hoechst 33342 as ‘dapi’) and all the FOVs are provided as separate .tiff files. Each FOV comprises 81–95 focal planes spaced 0.2 μm apart and has a size of 1024x1024 pixels.

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Karolinska Institutet

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magda.bienko@ki.se

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