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Immunofluorescence data of 700 host cell proteins in Sars-CoV2 infected vs non-infected VeroE6 cells

dataset
posted on 17.02.2022, 16:05 authored by Jayasankar Mohanakrishnan Kaimal, Ulrika Axelsson, Trang H. Le, Marianna Tampere, Hao Xu, Hanna Axelsson, Anna Bäckström, Duncan Njenda, Elisabeth Moussaud-Lamodière, Wei OuyangWei Ouyang, Carolina Oses Sepulveda, Brinton Seashore-Ludlow, Caroline Vernersson, Ali Mirazimi, Päivi Östling, Emma Lundberg, Charlotte Stadler

The data set contains ≈ 75,000 confocal images of mixed Vero E6 cell populations (SARS-CoV2 infected vs non-infected). Cells had been infected with the virus for 1 h and fixed 24 hour post infection in 96 well plates. Using immunofluorescence (4 colours) cells were stained for the SARS-CoV2 virus (red), the endoplasmic reticulum (yellow) and nucleus (blue) – same for all images in the data set and used as reference markers. On top of this, 700 different target proteins were stained – one per well (green). Images were acquired with an Opera Phoenix microscope at 63X, using 9 FoV and 3 z-planes per protein target. IN the original study, intensity differences and spatial re-distribution of the target proteins were evaluated in infected vs the non-infected cells. The image resource was generated and described in detail in this research article: (to be added).

Funding

KAW 2020.0182

History

Publisher

KTH Royal Institute of Technology

Access request email

charlotte.stadler@scilifelab.se