Dataset for "Serological and molecular study of Crimean-Congo hemorrhagic fever virus in cattle from selected districts in Uganda" _serology_25012021
Title: Serological and molecular study of Crimean-Congo hemorrhagic fever virus in cattle from selected districts in Uganda
Journal of Virological Methods, in press 27 January 2021
Background: Crimean-Congo Hemorrhagic Fever (CCHF) is a severe tick-borne viral hemorrhagic disease caused by Crimean-Congo Hemorrhagic Fever Virus (CCHFV) that poses serious public health challenges in many parts of Africa, Europe and Asia.
Methods: We examined 500 cattle sera samples from five districts for CCHFV antibodies using in-house and commercially available (IDVet) ELISA, Immunofluorescent assay (IFA) and Real-time polymerase chain reaction (RT-PCR).
Results: 500 cattle (73.8% females) were analyzed; CCHFV seropositivity was 12.6% (n = 63) and 75.0% (n = 375) with the in-house and IDVet ELISAs, respectively. Seropositivity was associated with geographical location, increasing age, being female, and having a higher tick burden. Twentyfour out of the 37 (64.8%) were seropositive for CCHFV using IFA and negative for virus on RT-PCR. The IFA results were more comparable to IDVet (κcoefficient = 0.88, p = <0.01) than to in-house (κcoefficient = 0.32, p = 0.02).
Conclusions: Our study confirmed the presence and high prevalence of anti-CCHF antibodies in cattle based on three methods from all the five study districts, confirming presence and exposure of CCHFV. Given the zoonotic potential for CCHFV, we recommend a multidisciplinary public health surveillance and epidemiology of CCHFV in both animals and humans throughout the country.